DNA Fingerprinting- Principle, Methods, Applications. September 4, 2022 by Sagar Aryal. Edited By: Sagar Aryal. DNA fingerprinting or DNA profiling is a process used to determine the nucleotide sequence at a certain part of the DNA that is unique in all human beings. The process of DNA fingerprinting was invented by Sir Alec Jeffrey at the
Furthermore, short paired-end reads with sequence overlaps can be assembled to create longer sequences, and assembled reads will span the full length of the original DNA fragment. So far, for STR analysis in whole-genome sequencing data, many tools have been developed, the most notable of which are LobSTR [ 16 ], HipSTR [ 17 ] and RepeatSeq [ 18 ].
Background Next generation sequencing (NGS) technology has revolutionized genomic and genetic research. The pace of change in this area is rapid with three major new sequencing platforms having been released in 2011: Ion Torrent’s PGM, Pacific Biosciences’ RS and the Illumina MiSeq. Here we compare the results obtained with those platforms to the performance of the Illumina HiSeq, the
Advantages of Targeted Gene Sequencing. Sequences key genes or regions of interest to high depth (500–1000× or higher), allowing identification of rare variants. Provides cost-effective findings for studies of disease-related genes. Delivers accurate, easy-to-interpret results, identifying variants at low allele frequencies (down to 5%)
Poly(A) tails at the 3’ end of eukaryotic messenger RNAs control mRNA stability and translation efficiency. Facilitated by various NGS methods, alternative polyadenylation sites determining the 3ʹ-UTR length of gene transcripts have been extensively studied. However, poly(A) lengths demonstrating dynamic and developmental regulation remain largely unexplored. The recently developed NGS
. 67 273 114 215 358 72 125 258
dna sequencing vs dna profiling
